Isozyme analysis of Plasmopara halstedii using cellulose-acetate gel electrophoresis
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چکیده
منابع مشابه
Haemoglobin electrophoresis on cellulose acetate using whole blood samples.
A modification of the method of haemoglobin electrophoresis using cellulose acetate membrane described by Kohn (1969) enables whole blood to be applied direct to the membrane instead of a haemolysate. The method gives results which are indistinguishable from those obtained using a haemolysate. Normal protein stains are not recommended, because of interference from plasma proteins, but the metho...
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A rapid, inexpensive, and quantitative method is described for obtaining the levels of plasma very low, low, and high density lipoproteins using cellulose acetate electrophoresis and lipid assays without prior separation by ultracentrifuge or other techniques. It involves separation of the lipoproteins by cellulose acetate electrophoresis, followed by their identification with the ozone-Schiff ...
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A method of electrophoresis utilizing cellulose acetate is described wherein quantitation of serum protein fractions by densitometry is accomplished within 2 hr. of initiation of the procedure. The densitometer was found to have a linear response to amido black dye. Albumin and y-globulin demonstrated equal dye-binding characteristics. Combined with the absence of albumin trailing, this has aff...
متن کاملPrediction of immunoglobulins from patterns of cellulose acetate electrophoresis.
The concentrations of serum immunoglobulins (Ig) C, A, and M were correlated with specific features in the $y region of densitometer patterns of serum protein electrophoresis on cellulose acetate membranes; visual analysis of densitometer patterns permitted semiquantitative prediction of values for lgC and lgA. Important immunoglobulin abnormalities, particularly elevations of IgA, were seen in...
متن کاملRapid electrophoresis and quantitation of haemoglobins on cellulose acetate.
A rapid and reproducible electrophoretic method for the separation and quantitation of haemoglobins on cellulose acetate is described. The accuracy of the method and its possible sources of error are discussed. The normal range for haemoglobin A(2) by this method is 1% to 3% of the total haemoglobin concentration. Blood samples from 32 thalassaemic patients showed haemoglobin A(2) values of 3.5...
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ژورنال
عنوان ژورنال: Plant Pathology
سال: 2007
ISSN: 0032-0862,1365-3059
DOI: 10.1111/j.1365-3059.2007.01737.x